IRESite_Id:57 summary record describing characteristics of AML1/RUNX1 IRES

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `57`	Version: `8`
Originaly submitted by: Václav Vopálenský	Submission date: `2006-01-12 00:00:00`
Reviewed by: Martin Mokrejš	Last change: `2010-11-30 21:33:39`

IRESite record type:

natural_transcript

The shape of the nucleic acid molecule translated: `linear`	The quality of the mRNA/+RNA sequence: `5UTR_possibly_incomplete`
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule: `AML1/RUNX1`	The genetic origin of this natural mRNA/+RNA: `nuclear`

The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
49574545

Synonyms of the gene name:

Synonym: AML1

Synonym: CBFA2

Synonym: EVI-1

Synonym: AMLCR1

Synonym: PEBP2A2

Synonym: PEBP2aB

Synonym: AML1-EVI-1

The mRNA/+RNA description:

Homo sapiens runt-related transcription factor 1 (acute myeloid leukemia 1; aml1 oncogene) (RUNX1), transcript
variant 2, mRNA. Poly(A)-tail sequence dropped.

The mRNA/+RNA sequence represented in the +DNA notation:

CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC CACAAACTTA ACCAACATCC CCAAACCCGA GTTCACAGAT GTGGGAGAGC 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

TGTAGAACCC TGAGTGTCAT CGACTGGGCC TTCTTATGAT TGTTGTTTTA AGATTAGCTG AAGATCTCTG AAACGCTGAA TTTTCTGCAC TGAGCGTTTT 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

GACAGAATTC ATTGAGAGAA CAGAGAACAT GACAAGTACT TCTAGCTCAG CACTGCTCCA ACTACTGAAG CTGATTTTCA AGGCTACTTA AAAAAATCTG 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CAGCGTACAT TAATGGATTT CTGTTGTGTT TAAATTCTCC ACAGATTGTA TTGTAAATAT TTTATGAAGT AGAGCATATG TATATATTTA TATATACGTG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

CACATACATT AGTAGCACTA CCTTTGGAAG TCTCAGCTCT TGCTTTTCGG GACTGAAGCC AGTTTTGCAT GATAAAAGTG GCCTTGTTAC GGGAGATAAT 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

TGTGTTCTGT TGGGACTTTA GACAAAACTC ACCTGCAAAA AACTGACAGG CATTAACTAC TGGAACTTCC AAATAATGTG TTTGCTGATC GTTTTACTCT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

TCGCATAAAT ATTTTAGGAA GTGTATGAGA ATTTTGCCTT CAGGAACTTT TCTAACAGCC AAAGACAGAA CTTAACCTCT GCAAGCAAGA TTCGTGGAAG 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

ATAGTCTCCA CTTTTTAATG CACTAAGCAA TCGGTTGCTA GGAGCCCATC CTGGGTCAGA GGCCGATCCG CAGAACCAGA ACGTTTTCCC CTCCTGGACT 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

GTTAGTAACT TAGTCTCCCT CCTCCCCTAA CCACCCCCGC CCCCCCCCAC CCCCCGCAGT AATAAAGGCC CCTGAACGTG TATGTTGGTC TCCCGGGAGC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TGCTTGCTGA AGATCCGCGC CCCTGTCGCC GTCTGGTAGG AGCTGTTTGC AGGGTCCTAA CTCAATCGGC TTGTTGTGAT GCGTATCCCC GTAGATGCCA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

GCACGAGCCG CCGCTTCACG CCGCCTTCCA CCGCGCTGAG CCCAGGCAAG ATGAGCGAGG CGTTGCCGCT GGGCGCCCCG GACGCCGGCG CTGCCCTGGC 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CGGCAAGCTG AGGAGCGGCG ACCGCAGCAT GGTGGAGGTG CTGGCCGACC ACCCGGGCGA GCTGGTGCGC ACCGACAGCC CCAACTTCCT CTGCTCCGTG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

CTGCCTACGC ACTGGCGCTG CAACAAGACC CTGCCCATCG CTTTCAAGGT GGTGGCCCTA GGGGATGTTC CAGATGGCAC TCTGGTCACT GTGATGGCTG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

GCAATGATGA AAACTACTCG GCTGAGCTGA GAAATGCTAC CGCAGCCATG AAGAACCAGG TTGCAAGATT TAATGACCTC AGGTTTGTCG GTCGAAGTGG 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

AAGAGGGAAA AGCTTCACTC TGACCATCAC TGTCTTCACA AACCCACCGC AAGTCGCCAC CTACCACAGA GCCATCAAAA TCACAGTGGA TGGGCCCCGA 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

GAACCTCGAA GACATCGGCA GAAACTAGAT GATCAGACCA AGCCCGGGAG CTTGTCCTTT TCCGAGCGGC TCAGTGAACT GGAGCAGCTG CGGCGCACAG 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

CCATGAGGGT CAGCCCACAC CACCCAGCCC CCACGCCCAA CCCTCGTGCC TCCCTGAACC ACTCCACTGC CTTTAACCCT CAGCCTCAGA GTCAGATGCA 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

GGATACAAGG CAGATCCAAC CATCCCCACC GTGGTCCTAC GATCAGTCCT ACCAATACCT GGGATCCATT GCCTCTCCTT CTGTGCACCC AGCAACGCCC 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

ATTTCACCTG GACGTGCCAG CGGCATGACA ACCCTCTCTG CAGAACTTTC CAGTCGACTC TCAACGGCAC CCGACCTGAC AGCGTTCAGC GACCCGCGCC 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

AGTTCCCCGC GCTGCCCTCC ATCTCCGACC CCCGCATGCA CTATCCAGGC GCCTTCACCT ACTCCCCGAC GCCGGTCACC TCGGGCATCG GCATCGGCAT 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

GTCGGCCATG GGCTCGGCCA CGCGCTACCA CACCTACCTG CCGCCGCCCT ACCCCGGCTC GTCGCAAGCG CAGGGAGGCC CGTTCCAAGC CAGCTCGCCC 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TCCTACCACC TGTACTACGG CGCCTCGGCC GGCTCCTACC AGTTCTCCAT GGTGGGCGGC GAGCGCTCGC CGCCGCGCAT CCTGCCGCCC TGCACCAACG 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

CCTCCACCGG CTCCGCGCTG CTCAACCCCA GCCTCCCGAA CCAGAGCGAC GTGGTGGAGG CCGAGGGCAG CCACAGCAAC TCCCCCACCA ACATGGCGCC 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

CTCCGCGCGC CTGGAGGAGG CCGTGTGGAG GCCCTACTGA GGCGCCAGGC CTGGCCCGGC TGGGCCCCGC GGGCCGCCGC CTTCGCCTCC GGGCGCGCGG 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

GCCTCCTGTT CGCGACAAGC CCGCCGGGAT CCCGGGCCCT GGGCCCGGCC ACCGTCCTGG GGCCGAGGGC GCCCGACGGC CAGGATCTCG CTGTAGGTCA 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

GGCCCGCGCA GCCTCCTGCG CCCAGAAGCC CACGCCGCCG CCGTCTGCTG GCGCCCCGGC CCTCGCGGAG GTGTCCGAGG CGACGCACCT CGAGGGTGTC 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

CGCCGGCCCC AGCACCCAGG GGACGCGCTG GAAAGCAAAC AGGAAGATTC CCGGAGGGAA ACTGTGAATG CTTCTGATTT AGCAATGCTG TGAATAAAAA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

GAAAGATTTT ATACCCTTGA CTTAACTTTT TAACCAAGTT GTTTATTCCA AAGAGTGTGG AATTTTGGTT GGGGTGGGGG GAGAGGAGGG ATGCAACTCG 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

CCCTGTTTGG CATCTAATTC TTATTTTTAA TTTTTCCGCA CCTTATCAAT TGCAAAATGC GTATTTGCAT TTGGGTGGTT TTTATTTTTA TATACGTTTA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

TATAAATATA TATAAATTGA GCTTGCTTCT TTCTTGCTTT GACCATGGAA AGAAATATGA TTCCCTTTTC TTTAAGTTTT ATTTAACTTT TCTTTTGGAC 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

TTTTGGGTAG TTGTTTTTTT TTGTTTTGTT TTGTTTTTTT GAGAAACAGC TACAGCTTTG GGTCATTTTT AACTACTGTA TTCCCACAAG GAATCCCCAG 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ATATTTATGT ATCTTGATGT TCAGACATTT ATGTGTTGAT AATTTTTTAA TTATTTAAAT GTACTTATAT TAAGAAAAAT ATCAAGTACT ACATTTTCTT 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TTGTTCTTGA TAGTAGCCAA AGTTAAATGT ATCACATTGA AGAAGGCTAG AAAAAAAGAA TGAGTAATGT GATCGCTTGG TTATCCAGAA GTATTGTTTA 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

CATTAAACTC CCTTTCATGT TAATCAAACA AGTGAGTAGC TCACGCAGCA ACGTTTTTAA TAGGATTTTT AGACACTGAG GGTCACTCCA AGGATCAGAA 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

GTATGGAATT TTCTGCCAGG CTCAACAAGG GTCTCATATC TAACTTCCTC CTTAAAACAG AGAAGGTCAA TCTAGTTCCA GAGGGTTGAG GCAGGTGCCA 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

ATAATTACAT CTTTGGAGAG GATTTGATTT CTGCCCAGGG ATTTGCTCAC CCCAAGGTCA TCTGATAATT TCACAGATGC TGTGTAACAG AACACAGCCA 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

AAGTAAACTG TGTAGGGGAG CCACATTTAC ATAGGAACCA AATCAATGAA TTTAGGGGTT ACGATTATAG CAATTTAAGG GCCACCAGAA GCAGGCCTCG 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

AGGAGTCAAT TTGCCTCTGT GTGCCTCAGT GGAGACAAGT GGGAAAACAT GGTCCCACCT GTGCGAGACC CCCTGTCCTG TGCTGCTCAC TCAACAACAT 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

CTTTGTGTTG CTTTCACCAG GCTGAGACCC TACCCTATGG GGTATATGGG CTTTTACCTG TGCACCAGTG TGACAGGAAA GATTCATGTC ACTACTGTCC 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

GTGGCTACAA TTCAAAGGTA TCCAATGTCG CTGTAAATTT TATGGCACTA TTTTTATTGG AGGATTTGGT CAGAATGCAG TTGTTGTACA ACTCATAAAT 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

ACTAACTGCT GATTTTGACA CATGTGTGCT CCAAATGATC TGGTGGTTAT TTAACGTACC TCTTAAAATT CGTTGAAACG ATTTCAGGTC AACTCTGAAG 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

AGTATTTGAA AGCAGGACTT CAGAACAGTG TTTGATTTTT ATTTTATAAA TTTAAGCATT CAAATTAGGC AAATCTTTGG CTGCAGGCAG CAAAAACAGC 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

TGGACTTATT TAAAACAACT TGTTTTTGAG TTTTCTTATA TATATATTGA TTATTTGTTT TACACACATG CAGTAGCACT TTGGTAAGAG TTAAAGAGTA 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

AAGCAGCTTA TGTTGTCAGG TCGTTCTTAT CTAGAGAAGA GCTATAGCAG ATCTCGGACA AACTCAGAAT ATATTCACTT TCATTTTTGA CAGGATTCCC 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

TCCACAACTC AGTTTCATAT ATTATTCCGT ATTACATTTT TGCAGCTAAA TTACCATAAA ATGTCAGCAA ATGTAAAAAT TTAATTTCTG AAAAGCACCA 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

TTAGCCCATT TCCCCCAAAT TAAACGTAAA TGTTTTTTTT CAGCACATGT TACCATGTCT GACCTGCAAA AATGCTGGAG AAAAATGAAG GAAAAAATTA 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

TGTTTTTCAG TTTAATTCTG TTAACTGAAG ATATTCCAAC TCAAAACCAG CCTCATGCTC TGATTAGATA ATCTTTTACA TTGAACCTTT ACTCTCAAAG 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

CCATGTGTGG AGGGGGCTTG TCACTATTGT AGGCTCACTG GATTGGTCAT TTAGAGTTTC ACAGACTCTT ACCAGCATAT ATAGTATTTA ATTGTTTCAA 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

AAAAAATCAA ACTGTAGTTG TTTTGGCGAT AGGTCTCACG CAACACATTT TTGTATGTGT GTGTGTGTGC GTGTGTGTGT GTGTGTGTGA AAAATTGCAT 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

TCATTGACTT CAGGTAGATT AAGGTATCTT TTTATTCATT GCCCTCAGGA AAGTTAAGGT ATCAATGAGA CCCTTAAGCC AATCATGTAA TAACTGCATG 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

TGTCTGGTCC AGGAGAAGTA TTGAATAAGC CATTTCTACT GCTTACTCAT GTCCCTATTT ATGATTTCAA CATGGATACA TATTTCAGTT CTTTCTTTTT 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

CTCACTATCT GAAAATACAT TTCCCTCCCT CTCTTCCCCC CAATATCTCC CTTTTTTTCT CTCTTCCTCT ATCTTCCAAA CCCCACTTTC TCCCTCCTCC 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

TTTTCCTGTG TTCTCTTAAG CAGATAGCAC ATACCCCCAC CCAGTACCAA ATTTCAGAAC ACAAGAAGGT CCAGTTCTTC CCCCTTCACA TAAAGGAACA 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

TGGTTTGTCA GCCTTTCTCC TGTTTATGGG TTTCTTCCAG CAGAACAGAG ACATTGCCAA CCATATTGGA TCTGCTTGCT GTCCAAACCA GCAAACTTTC 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

CTGGGCAAAT CACAATCAGT GAGTAAATAG ACAGCCTTTC TGCTGCCTTG GGTTTCTGTG CAGATAAACA GAAATGCTCT GATTAGAAAG GAAATGAATG 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

GTTCCACTCA AATGTCCTGC AATTTAGGAT TGCAGATTTC TGCCTTGAAA TACCTGTTTC TTTGGGACAT TCCGTCCTGA TGATTTTTAT TTTTGTTGGT 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

TTTTATTTTT GGGGGGAATG ACATGTTTGG GTCTTTTATA CATGAAAATT TGTTTGACAA TAATCTCACA AAACATATTT TACATCTGAA CAAAATGCCT 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

TTTTGTTTAC CGTAGCGTAT ACATTTGTTT TGGGATTTTT GTGTGTTTGT TGGGAATTTT GTTTTTAGCC AGGTCAGTAT TGATGAGGCT GATCATTTGG 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

CTCTTTTTTT CCTTCCAGAA GAGTTGCATC AACAAAGTTA ATTGTATTTA TGTATGTAAA TAGATTTTAA GCTTCATTAT AAAATATTGT TAATGCCTAT 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

AACTTTTTTT CAATTTTTTT GTGTGTGTTT CTAAGGACTT TTTCTTAGGT TTGCTAAATA CTGTAGGGAA AAAAATGCTT CTTTCTACTT TGTTTATTTT 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

AGACTTTAAA ATGAGCTACT TCTTATTCAC TTTTGTAAAC AGCTAATAGC ATGGTTCCAA TTTTTTTTAA GTTCACTTTT TTTGTTCTAG GGGAAATGAA 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

TGTGCAAAAA AAGAAAAAGA ACTGTTGGTT ATTTGTGTTA TTCTGGATGT ATAAAAATCA ATGGAAAAAA ATAAACTTTC AAATTGAAAT GACGGTATAA 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

CACATCTACT GAAAAAGCAA CGGGAAATGT GGTCCTATTT AAGCCAGCCC CCACCTAGGG TCTATTTGTG TGGCAGTTAT TGGGTTTGGT CACAAAACAT 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

CCTGAAAATT CGTGCGTGGG CTTCTTTCTC CCTGGTACAA ACGTATGGAA TGCTTCTTAA AGGGGAACTG TCAAGCTGGT GTCTTCAGCC AGATGACATG 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

AGAGAATATC CCAGAACCCT CTCTCCAAGG TGTTTCTAGA TAGCACAGGA GAGCAGGCAC TGCACTGTCC ACAGTCCACG GTACACAGTC GGGTGGGCCG 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

CCTCCCCTCT CCTGGGAGCA TTCGTCGTGC CCAGCCTGAG CAGGGCAGCT GGACTGCTGC TGTTCAGGAG CCACCAGAGC CTTCCTCTCT TTGTACCACA 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

GTTTCTTCTG TAAATCCAGT GTTACAATCA GTGTGAATGG CAAATAAACA GTTTGACAAG TACATACACC AT
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271

Credibility of mRNA sequence:

guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail

The organism containing this mRNA with IRES segment in its genome:

Homo sapiens

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
1

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `2`	Last change: `2009-09-01 17:37:18`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
RUNX1

The description of the protein encoded in this ORF:
runt-related transcription factor 1 isoform b

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1579-2940

Remarks:

Expression of AML1/RUNX1 is regulated at the level of transcription by two promoters distal (D) and proximal
(P) located about 150kb apart. While the transcripts with shorter (452b) D-UTR (6 kb in total) are efficiently
translated by cap-dependent translation (95% compared to CAT 5'-UTR and reverse-oriented D-UTR in
monocistronic assay), the longer P-UTR variant (1631b) transcripts (3, 4 and 8 kb in total) are poorly
translated (31% compared to CAT 5'-UTR) and translation initiation is mediated by a cap-independent
translation through IRES.
The P-UTR (1631b) is from a single exon and contains 15 uAUG codons and two GC-rich islands. The D-UTR (452b)
is spliced from 4 exons and the protein encoded is longer by 32 aminoacids at its N-terminus in contrast to
the proteins encoded by 4 and 8kb mRNAs. It depends on the cell line whether the trancripts with longer or
shorter 5'-UTR are more abundant (which reflects activity of the proximal or distal promoter). It seems both
promoters are active simultaneously, although to various extent.

AML/RUNX genes (previously termed CBFA or PEBP2alpha) belong to a family of heterodimeric transcription
factors having 128aa runt domain (RD), because of its homology to Drosophila Runt protein. AML1/RUNX1 and
AML3/RUNX2 play crucial role in hematopoiesis in mice while AML3/RUNX2 is essential for osteoporesis.

5'-UTR is incomplete, according to the article should have 1631b instead of only 1578 available here
(GI:966996).
P-UTR contains UUUCC sequence in its 3' part and complementary to the 3' end of 18S rRNA and further it
contains internal oligopyrimidine tract.

AML1 IRES was studied using capped T7 transcripts in Rabbit Reticulocyte Lysates and their translation was
resistant to pretreatment (30 min) of RRL by picornaviral 2A protease cleaving eIF4G protein.

Integrity of the transcripts was verified by Northern blot utilising 590b long probe against 5' part of the
second cistron (LUC) of BAP plasmid.

Citations:

Pozner A., Goldenberg D., Negreanu V., Le S. Y., Elroy-Stein O., Levanon D., Groner Y. (2000) Transcription-coupled translation control of AML1/RUNX1 is mediated by cap- and internal ribosome entry site-dependent mechanisms. Mol. Cell. Biol. 20(7):2297-2307

Bee T, Ashley EL, Bickley SR, Jarratt A, Li PS, Sloane-Stanley J, Göttgens B, de Bruijn MF (2009) The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. Blood. 113(21):5121-5124

IRESs:

IRES:

Version: `3`	Last change: `2007-01-19 00:00:00`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `AML1/RUNX1`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

39-1599

Conclusion:
putative_IRES

How IRES boundaries were determined:
guessed

The sequence of IRES region aligned to its secondary structure (if available):

GGCCGGACCC AGCCAGGGCA CCACGCTGCC CGGCCCTGCG CCGCCAGGCA CTTCTTTCCG GGGCTCCTAG GGACGCCAGA AGGAAGTCAA CCTCTGCTGC 
^39        ^49        ^59        ^69        ^79        ^89        ^99        ^109       ^119       ^129       

TTCTCCTTGG CCTGCGTTGG ACCTTCCTTT TTTTGTTGTT TTTTTTTGTT TTTCCCCTTT CTTCCTTTTG AATTAACTGG CTTCTTGGCT GGATGTTTTC 
^139       ^149       ^159       ^169       ^179       ^189       ^199       ^209       ^219       ^229       

AACTTCTTTC CTGGCTGCGA ACTTTTCCCC AATTGTTTTC CTTTTACAAC AGGGGGAGAA AGTGCTCTGT GGTCCGAGGC GAGCCGTGAA GTTGCGTGTG 
^239       ^249       ^259       ^269       ^279       ^289       ^299       ^309       ^319       ^329       

CGTGGCAGTG TGCGTGGCAG GATGTGCGTG CGTGTGTAAC CCGAGCCGCC CGATCTGTTT CGATCTGCGC CGCGGAGCCC TCCCTCAAGG CCCGCTCCAC 
^339       ^349       ^359       ^369       ^379       ^389       ^399       ^409       ^419       ^429       

CTGCTGCGGT TACGCGGCGC TCGTGGGTGT TCGTGCCTCG GAGCAGCTAA CCGGCGGGTG CTGGGCGACG GTGGAGGAGT ATCGTCTCGC TGCTGCCCGA 
^439       ^449       ^459       ^469       ^479       ^489       ^499       ^509       ^519       ^529       

GTCAGGGCTG AGTCACCCAG CTGATGTAGA CAGTGGCTGC CTTCCGAAGA GTGCGTGTTT GCATGTGTGT GACTCTGCGG CTGCTCAACT CCCAACAAAC 
^539       ^549       ^559       ^569       ^579       ^589       ^599       ^609       ^619       ^629       

CAGAGGACCA GCCACAAACT TAACCAACAT CCCCAAACCC GAGTTCACAG ATGTGGGAGA GCTGTAGAAC CCTGAGTGTC ATCGACTGGG CCTTCTTATG 
^639       ^649       ^659       ^669       ^679       ^689       ^699       ^709       ^719       ^729       

ATTGTTGTTT TAAGATTAGC TGAAGATCTC TGAAACGCTG AATTTTCTGC ACTGAGCGTT TTGACAGAAT TCATTGAGAG AACAGAGAAC ATGACAAGTA 
^739       ^749       ^759       ^769       ^779       ^789       ^799       ^809       ^819       ^829       

CTTCTAGCTC AGCACTGCTC CAACTACTGA AGCTGATTTT CAAGGCTACT TAAAAAAATC TGCAGCGTAC ATTAATGGAT TTCTGTTGTG TTTAAATTCT 
^839       ^849       ^859       ^869       ^879       ^889       ^899       ^909       ^919       ^929       

CCACAGATTG TATTGTAAAT ATTTTATGAA GTAGAGCATA TGTATATATT TATATATACG TGCACATACA TTAGTAGCAC TACCTTTGGA AGTCTCAGCT 
^939       ^949       ^959       ^969       ^979       ^989       ^999       ^1009      ^1019      ^1029      

CTTGCTTTTC GGGACTGAAG CCAGTTTTGC ATGATAAAAG TGGCCTTGTT ACGGGAGATA ATTGTGTTCT GTTGGGACTT TAGACAAAAC TCACCTGCAA 
^1039      ^1049      ^1059      ^1069      ^1079      ^1089      ^1099      ^1109      ^1119      ^1129      

AAAACTGACA GGCATTAACT ACTGGAACTT CCAAATAATG TGTTTGCTGA TCGTTTTACT CTTCGCATAA ATATTTTAGG AAGTGTATGA GAATTTTGCC 
^1139      ^1149      ^1159      ^1169      ^1179      ^1189      ^1199      ^1209      ^1219      ^1229      

TTCAGGAACT TTTCTAACAG CCAAAGACAG AACTTAACCT CTGCAAGCAA GATTCGTGGA AGATAGTCTC CACTTTTTAA TGCACTAAGC AATCGGTTGC 
^1239      ^1249      ^1259      ^1269      ^1279      ^1289      ^1299      ^1309      ^1319      ^1329      

TAGGAGCCCA TCCTGGGTCA GAGGCCGATC CGCAGAACCA GAACGTTTTC CCCTCCTGGA CTGTTAGTAA CTTAGTCTCC CTCCTCCCCT AACCACCCCC 
^1339      ^1349      ^1359      ^1369      ^1379      ^1389      ^1399      ^1409      ^1419      ^1429      

GCCCCCCCCC ACCCCCCGCA GTAATAAAGG CCCCTGAACG TGTATGTTGG TCTCCCGGGA GCTGCTTGCT GAAGATCCGC GCCCCTGTCG CCGTCTGGTA 
^1439      ^1449      ^1459      ^1469      ^1479      ^1489      ^1499      ^1509      ^1519      ^1529      

GGAGCTGTTT GCAGGGTCCT AACTCAATCG GCTTGTTGTG ATGCGTATCC CCGTAGATGC C
^1539      ^1549      ^1559      ^1569      ^1579      ^1589      ^1599

Remarks:

Part of the AML1/RUNX1 5' UTR (nt 39 to nt 1578) and the first 21 nt from ORF.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1