IRESite_Id:610 characteristics of crTMV_IRESmp75 IRES studied in H-GFP-crTMV

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `610`	Version: `2`
Originaly submitted by: Martin Mokrejš	Submission date: `2009-08-25 16:51:07`
Reviewed by: Martin Mokrejš	Last change: `2009-08-25 21:07:59`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
both_UTRs_incomplete

The mRNA/+RNA description:

Part of the T7-derived transcript studied in some in vitro assays. The 5'-UTR could have been longer but we do
not know the necessary details. The intercistronic region contains sequence preceding movement protein (MP)
ORF in crTMV (bp -75 to -3).

The mRNA/+RNA sequence represented in the +DNA notation:

ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCACCTA 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCACGGCA 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

TGGACGAGCT GTACAAGTAA gcttcgtttg ctttttgtag tataattaaa tatttgtcag ataagagatt ggttagagat ttgttctttg tttgaccATG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

GTCCGTCCTG TAGAAACCCC AACCCGTGAA ATCAAAAAAC TCGACGGCCT GTGGGCATTC AGTCTGGATC GCGAAAACTG TGGAATTGAT CAGCGTTGGT 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

GGGAAAGCGC GTTACAAGAA AGCCGGGCAA TTGCTGTGCC AGGCAGTTTT AACGATCAGT TCGCCGATGC AGATATTCGT AATTATGCGG GCAACGTCTG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

GTATCAGCGC GAAGTCTTTA TACCGAAAGG TTGGGCAGGC CAGCGTATCG TGCTGCGTTT CGATGCGGTC ACTCATTACG GCAAAGTGTG GGTCAATAAT 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CAGGAAGTGA TGGAGCATCA GGGCGGCTAT ACGCCATTTG AAGCCGATGT CACGCCGTAT GTTATTGCCG GGAAAAGTGT ACGTATCACC GTTTGTGTGA 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ACAACGAACT GAACTGGCAG ACTATCCCGC CGGGAATGGT GATTACCGAC GAAAACGGCA AGAAAAAGCA GTCTTACTTC CATGATTTCT TTAACTATGC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

CGGAATCCAT CGCAGCGTAA TGCTCTACAC CACGCCGAAC ACCTGGGTGG ACGATATCAC CGTGGTGACG CATGTCGCGC AAGACTGTAA CCACGCGTCT 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

GTTGACTGGC AGGTGGTGGC CAATGGTGAT GTCAGCGTTG AACTGCGTGA TGCGGATCAA CAGGTGGTTG CAACTGGACA AGGCACTAGC GGGACTTTGC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

AAGTGGTGAA TCCGCACCTC TGGCAACCGG GTGAAGGTTA TCTCTATGAA CTGTGCGTCA CAGCCAAAAG CCAGACAGAG TGTGATATCT ACCCGCTTCG 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CGTCGGCATC CGGTCAGTGG CAGTGAAGGG CGAACAGTTC CTGATTAACC ACAAACCGTT CTACTTTACT GGCTTTGGTC GTCATGAAGA TGCGGACTTA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CGTGGCAAAG GATTCGATAA CGTGCTGATG GTGCACGACC ACGCATTAAT GGACTGGATT GGGGCCAACT CCTACCGTAC CTCGCATTAC CCTTACGCTG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

AAGAGATGCT CGACTGGGCA GATGAACATG GCATCGTGGT GATTGATGAA ACTGCTGCTG TCGGCTTTAA CCTCTCTTTA GGCATTGGTT TCGAAGCGGG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CAACAAGCCG AAAGAACTGT ACAGCGAAGA GGCAGTCAAC GGGGAAACTC AGCAAGCGCA CTTACAGGCG ATTAAAGAGC TGATAGCGCG TGACAAAAAC 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CACCCAAGCG TGGTGATGTG GAGTATTGCC AACGAACCGG ATACCCGTCC GCAAGGTGCA CGGGAATATT TCGCGCCACT GGCGGAAGCA ACGCGTAAAC 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TCGACCCGAC GCGTCCGATC ACCTGCGTCA ATGTAATGTT CTGCGACGCT CACACCGATA CCATCAGCGA TCTCTTTGAT GTGCTGTGCC TGAACCGTTA 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

TTACGGATGG TATGTCCAAA GCGGCGATTT GGAAACGGCA GAGAAGGTAC TGGAAAAAGA ACTTCTGGCC TGGCAGGAGA AACTGCATCA GCCGATTATC 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ATCACCGAAT ACGGCGTGGA TACGTTAGCC GGGCTGCACT CAATGTACAC CGACATGTGG AGTGAAGAGT ATCAGTGTGC ATGGCTGGAT ATGTATCACC 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

GCGTCTTTGA TCGCGTCAGC GCCGTCGTCG GTGAACAGGT ATGGAATTTC GCCGATTTTG CGACCTCGCA AGGCATATTG CGCGTTGGCG GTAACAAGAA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

AGGGATCTTC ACTCGCGACC GCAAACCGAA GTCGGCGGCT TTTCTGCTGC AAAAACGCTG GACTGGCATG AACTTCGGTG AAAAACCGCA GCAGGGAGGC 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

AAACAATGA
^2601

Warning: available plasmid sequence is exactly same as the mRNA sequence. It seems we never managed to reconstruct the original plasmid sequence used to express the studied mRNA.

Credibility of mRNA sequence:

only_fragment_published_or_from_author_and_the_rest_is_a_guess

The name of the plasmid:
H-GFP-crTMV_IRESmp75-GUS The name of the promoter used to express this mRNA:
T7

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
crTMV

The origin of IRES in the plasmid:

viral

The donor organism of the IRES segment:

Crucifer tobamovirus

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCACCTA 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCACGGCA 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

TGGACGAGCT GTACAAGTAA gcttcgtttg ctttttgtag tataattaaa tatttgtcag ataagagatt ggttagagat ttgttctttg tttgaccATG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

GTCCGTCCTG TAGAAACCCC AACCCGTGAA ATCAAAAAAC TCGACGGCCT GTGGGCATTC AGTCTGGATC GCGAAAACTG TGGAATTGAT CAGCGTTGGT 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

GGGAAAGCGC GTTACAAGAA AGCCGGGCAA TTGCTGTGCC AGGCAGTTTT AACGATCAGT TCGCCGATGC AGATATTCGT AATTATGCGG GCAACGTCTG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

GTATCAGCGC GAAGTCTTTA TACCGAAAGG TTGGGCAGGC CAGCGTATCG TGCTGCGTTT CGATGCGGTC ACTCATTACG GCAAAGTGTG GGTCAATAAT 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CAGGAAGTGA TGGAGCATCA GGGCGGCTAT ACGCCATTTG AAGCCGATGT CACGCCGTAT GTTATTGCCG GGAAAAGTGT ACGTATCACC GTTTGTGTGA 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ACAACGAACT GAACTGGCAG ACTATCCCGC CGGGAATGGT GATTACCGAC GAAAACGGCA AGAAAAAGCA GTCTTACTTC CATGATTTCT TTAACTATGC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

CGGAATCCAT CGCAGCGTAA TGCTCTACAC CACGCCGAAC ACCTGGGTGG ACGATATCAC CGTGGTGACG CATGTCGCGC AAGACTGTAA CCACGCGTCT 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

GTTGACTGGC AGGTGGTGGC CAATGGTGAT GTCAGCGTTG AACTGCGTGA TGCGGATCAA CAGGTGGTTG CAACTGGACA AGGCACTAGC GGGACTTTGC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

AAGTGGTGAA TCCGCACCTC TGGCAACCGG GTGAAGGTTA TCTCTATGAA CTGTGCGTCA CAGCCAAAAG CCAGACAGAG TGTGATATCT ACCCGCTTCG 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CGTCGGCATC CGGTCAGTGG CAGTGAAGGG CGAACAGTTC CTGATTAACC ACAAACCGTT CTACTTTACT GGCTTTGGTC GTCATGAAGA TGCGGACTTA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CGTGGCAAAG GATTCGATAA CGTGCTGATG GTGCACGACC ACGCATTAAT GGACTGGATT GGGGCCAACT CCTACCGTAC CTCGCATTAC CCTTACGCTG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

AAGAGATGCT CGACTGGGCA GATGAACATG GCATCGTGGT GATTGATGAA ACTGCTGCTG TCGGCTTTAA CCTCTCTTTA GGCATTGGTT TCGAAGCGGG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CAACAAGCCG AAAGAACTGT ACAGCGAAGA GGCAGTCAAC GGGGAAACTC AGCAAGCGCA CTTACAGGCG ATTAAAGAGC TGATAGCGCG TGACAAAAAC 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CACCCAAGCG TGGTGATGTG GAGTATTGCC AACGAACCGG ATACCCGTCC GCAAGGTGCA CGGGAATATT TCGCGCCACT GGCGGAAGCA ACGCGTAAAC 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TCGACCCGAC GCGTCCGATC ACCTGCGTCA ATGTAATGTT CTGCGACGCT CACACCGATA CCATCAGCGA TCTCTTTGAT GTGCTGTGCC TGAACCGTTA 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

TTACGGATGG TATGTCCAAA GCGGCGATTT GGAAACGGCA GAGAAGGTAC TGGAAAAAGA ACTTCTGGCC TGGCAGGAGA AACTGCATCA GCCGATTATC 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ATCACCGAAT ACGGCGTGGA TACGTTAGCC GGGCTGCACT CAATGTACAC CGACATGTGG AGTGAAGAGT ATCAGTGTGC ATGGCTGGAT ATGTATCACC 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

GCGTCTTTGA TCGCGTCAGC GCCGTCGTCG GTGAACAGGT ATGGAATTTC GCCGATTTTG CGACCTCGCA AGGCATATTG CGCGTTGGCG GTAACAAGAA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

AGGGATCTTC ACTCGCGACC GCAAACCGAA GTCGGCGGCT TTTCTGCTGC AAAAACGCTG GACTGGCATG AACTTCGGTG AAAAACCGCA GCAGGGAGGC 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

AAACAATGA
^2601

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
GFP

The description of the protein encoded in this ORF:
green fluorescent protein

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1-720

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
GUS

The description of the protein encoded in this ORF:
beta-glucuronidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

798-2609

Remarks:

The intercistronic sequence reveals that the crTMV genomic sequence in Z29370 probably contains a single-base
sequencing error.

Citations:

Dorokhov YL, Skulachev MV, Ivanov PA, Zvereva SD, Tjulkina LG, Merits A, Gleba YY, Hohn T, Atabekov JG (2002) Polypurine (A)-rich sequences promote cross-kingdom conservation of internal ribosome entry. Proc. Natl. Acad. Sci. U.S.A. 8(99):5301-5306

IRESs:

IRES:

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `crTMV_IRESmp75`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

723-795

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `3`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `75`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-75`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-3`

The sequence of IRES region aligned to its secondary structure (if available):

ttcgtttgct ttttgtagta taattaaata tttgtcagat aagagattgg ttagagattt gttctttgtt tga
^723       ^733       ^743       ^753       ^763       ^773       ^783       ^793

Citations:

The translation experiments:

Translation results:

IRESite Translation Id: 645

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vivo

The organism used for translation:

Homo sapiens HeLa (ATCC CCL-2)

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

28.600

Name of IRES used as the positive control:

EMCV-R

Name of the plasmid used as the positive control. `H-GFP-IRESemcv-GUS`
`IRESite Id` of the plasmid used as positive control. `608`
The relative translation efficiency in % of the positive control: `100.000`
The size (length) of intercistronic region in the positive control: `595`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

endogenous_cytoplasmic_uncapped_T7_transcript_with_polyA_tail

Remarks:

Data from Figure 2B. The cells were infected with recombinant vaccinia virus.

Citations:

Translation results:

IRESite Translation Id: 646

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vitro The in vitro translation system:
wheat germ lysate

The organism used for translation:

The temperature (in degrees of Celsia):
25

The relative translation efficiency in % of this IRES:

104.000

Name of IRES used as the positive control:

EMCV-R

Name of the plasmid used as the positive control. `H-GFP-IRESemcv-GUS`
`IRESite Id` of the plasmid used as positive control. `608`
The relative translation efficiency in % of the positive control: `100.000`
The size (length) of intercistronic region in the positive control: `595`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

exogenous_RNA_without_cap_without_polyA_tail

Remarks:

Data from Figure 2D.

Citations:

Translation results:

IRESite Translation Id: 647

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vitro The in vitro translation system:
rabbit reticulocytes lysate

The organism used for translation:

The temperature (in degrees of Celsia):
30

The relative translation efficiency in % of this IRES:

13.000

Name of IRES used as the positive control:

EMCV-R

Name of the plasmid used as the positive control. `H-GFP-IRESemcv-GUS`
`IRESite Id` of the plasmid used as positive control. `608`
The relative translation efficiency in % of the positive control: `100.000`
The size (length) of intercistronic region in the positive control: `595`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

exogenous_RNA_without_cap_without_polyA_tail

Remarks:

Data from Figure 2E.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1