IRESite_Id:85 summary record describing characteristics of AT1R

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `85`	Version: `4`
Originaly submitted by: Václav Vopálenský	Submission date: `2005-10-26 00:00:00`
Reviewed by: Martin Mokrejš	Last change: `2006-08-10 00:00:00`

IRESite record type:

natural_transcript

The shape of the nucleic acid molecule translated: `linear`	The quality of the mRNA/+RNA sequence: `end-to-end_full-length_mRNA`
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule: `hAT1R-D`	The genetic origin of this natural mRNA/+RNA: `nuclear`

The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
14043063

Synonyms of the gene name:

Synonym: AGTR1

Synonym: AG2S

Synonym: AT1B

Synonym: AT2R1

Synonym: HAT1R

Synonym: AGTR1A

Synonym: AGTR1B

Synonym: AT2R1A

Synonym: AT2R1B

Synonym: AT1

The mRNA/+RNA description:

Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 4, mRNA.

The mRNA/+RNA sequence represented in the +DNA notation:

ATTCGGAGCT GCCTCCTCGC CAATGATTCC AGCGCCTGAC AGCCAGGACC CCAGGCAGCA GCGAGTGACA GGACGTCTGG ACCGGCGCGC CGCTAGCAGC 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

TCTGCCGGGC CGCGGCGGTG ATCGATGGGG AGCGGCTGGA GCGGACCCAG CGAGTGAGGG CGCACAGCCG GGACGCCGAG GCGGCGGGCG GGAGACCCGC 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

ACCAGCGCAG CCGGCCCTCG GCGGGACGTG ACGCAGCGCC CGGGGCGCGG GTTTGATATT TGACAAATTG ATCTAAAATG GCTGGGTTTT TATCTGAATA 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

ACTCACTGAT GCCATCCCAG AAAGTCGGCA CCAGATGAAG AAAATGAATC ACAAGTCAAC TGACAGTCCA AAGGCTCCAC AGCTCAGAGG AGGTGTATTT 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

GATATAGTGT TTGCAACAAA TTCGACCCAG GTGATCAAAA TGATTCTCAA CTCTTCTACT GAAGATGGTA TTAAAAGAAT CCAAGATGAT TGTCCCAAAG 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CTGGAAGGCA TAATTACATA TTTGTCATGA TTCCTACTTT ATACAGTATC ATCTTTGTGG TGGGAATATT TGGAAACAGC TTGGTGGTGA TAGTCATTTA 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

CTTTTATATG AAGCTGAAGA CTGTGGCCAG TGTTTTTCTT TTGAATTTAG CACTGGCTGA CTTATGCTTT TTACTGACTT TGCCACTATG GGCTGTCTAC 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ACAGCTATGG AATACCGCTG GCCCTTTGGC AATTACCTAT GTAAGATTGC TTCAGCCAGC GTCAGTTTCA ACCTGTACGC TAGTGTGTTT CTACTCACGT 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

GTCTCAGCAT TGATCGATAC CTGGCTATTG TTCACCCAAT GAAGTCCCGC CTTCGACGCA CAATGCTTGT AGCCAAAGTC ACCTGCATCA TCATTTGGCT 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

GCTGGCAGGC TTGGCCAGTT TGCCAGCTAT AATCCATCGA AATGTATTTT TCATTGAGAA CACCAATATT ACAGTTTGTG CTTTCCATTA TGAGTCCCAA 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

AATTCAACCC TTCCGATAGG GCTGGGCCTG ACCAAAAATA TACTGGGTTT CCTGTTTCCT TTTCTGATCA TTCTTACAAG TTATACTCTT ATTTGGAAGG 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CCCTAAAGAA GGCTTATGAA ATTCAGAAGA ACAAACCAAG AAATGATGAT ATTTTTAAGA TAATTATGGC AATTGTGCTT TTCTTTTTCT TTTCCTGGAT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

TCCCCACCAA ATATTCACTT TTCTGGATGT ATTGATTCAA CTAGGCATCA TACGTGACTG TAGAATTGCA GATATTGTGG ACACGGCCAT GCCTATCACC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

ATTTGTATAG CTTATTTTAA CAATTGCCTG AATCCTCTTT TTTATGGCTT TCTGGGGAAA AAATTTAAAA GATATTTTCT CCAGCTTCTA AAATATATTC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

CCCCAAAAGC CAAATCCCAC TCAAACCTTT CAACAAAAAT GAGCACGCTT TCCTACCGCC CCTCAGATAA TGTAAGCTCA TCCACCAAGA AGCCTGCACC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

ATGTTTTGAG GTTGAGTGAC ATGTTCGAAA CCTGTCCATA AAGTAATTTT GTGAAAGAAG GAGCAAGAGA ACATTCCTCT GCAGCACTTC ACTACCAAAT 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

GAGCATTAGC TACTTTTCAG AATTGAAGGA GAAAATGCAT TATGTGGACT GAACCGACTT TTCTAAAGCT CTGAACAAAA GCTTTTCTTT CCTTTTGCAA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CAAGACAAAG CAAAGCCACA TTTTGCATTA GACAGATGAC GGCTGCTCGA AGAACAATGT CAGAAACTCG ATGAATGTGT TGATTTGAGA AATTTTACTG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ACAGAAATGC AATCTCCCTA GCCTGCTTTT GTCCTGTTAT TTTTTATTTC CACATAAAGG TATTTAGAAT ATATTAAATC GTTAGAGGAG CAACAGGAGA 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

TGAGAGTTCC AGATTGTTCT GTCCAGTTTC CAAAGGGCAG TAAAGTTTTC GTGCCGGTTT TCAGCTATTA GCAACTGTGC TACACTTGCA CCTGGTACTG 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CACATTTTGT ACAAAGATAT GCTAAGCAGT AGTCGTCAAG TTGCAGATCT TTTTGTGAAA TTCAACCTGT GTCTTATAGG TTTACACTGC CAAAACAATG 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

CCCGTAAGAT GGCTTATTTG TATAATGGTG TTACTAAAGT CACATATAAA AGTTAAACTA CTTGTAAAGG TGCTGCACTG GTCCCAAGTA GTAGTGTCCT 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

CCTAGTATAT TAGTTTGATT TAATATCTGA GAAGTGTATA TAGTTTGTGG TAAAAAGATT ATATATCATA AAGTATGCCT TCCTGTTTAA AAAAAGTATA 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

TATTCTACAC ATATATATAT ATGTATATCT ATATCTCTAA ACTGCTGTTA ATTGATTAAA ATCTGGCAAA GTTATATTTA CTTTAAAATA AAATAATTTT 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

ATTGC
^2401

Credibility of mRNA sequence:

guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail

The organism containing this mRNA with IRES segment in its genome:

Homo sapiens

A promoter reported in cDNA corresponding to IRES sequence:
no The total number of notable open-reading frames (ORFs):
1

Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:

Summary of experiments studying integrity of the in vivo transcripts in a particular host:

Integrity (uniformity) of mRNA tested using Northern-blot:
homogeneous_population_of_molecules_confirmed

Integrity (uniformity) of mRNA tested using RNase protection:
not_tested

Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested

Integrity (uniformity) of mRNA tested using primer extension :
not_tested

Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested

Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested

Integrity (uniformity) of mRNA tested using RNAi:
not_tested

Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested

Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
no_promoter_confirmed

Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested

Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested

The organism used:

Homo sapiens NCI-H295 [H295] [H295-R] (ATCC CRL-10296)

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `2`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
AGTR1

The description of the protein encoded in this ORF:
angiotensin II receptor, type 1

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

440-1519

OPTIONAL: The function of the encoded protein

GO:0005886; Cellular component: plasma membrane
GO:0016021; Cellular component: integral to membrane
GO:0005887; Cellular component: integral to plasma membrane
GO:0004872; Molecular function: receptor activity
GO:0004947; Molecular function: bradykinin receptor activity
GO:0001584; Molecular function: rhodopsin-like receptor activity
GO:0016494; Molecular function: C-X-C chemokine receptor activity
GO:0004945; Molecular function: angiotensin type II receptor activity
GO:0007589; Biological process: fluid secretion
GO:0008015; Biological process: circulation
GO:0007165; Biological process: signal transduction
GO:0007204; Biological process: elevation of cytosolic calcium ion concentration
GO:0007186; Biological process: G-protein coupled receptor protein signaling pathway
GO:0007200; Biological process: G-protein signaling, coupled to IP3 second messenger (phospholipase C
activating)

Remarks:

Integrity of the bicistronic transcripts transcribed in vivo was verified by Northern blot analysis using cDNA
probes against firefly luciferase. Cryptic promoter existence within the AT1R 5'-UTR was ruled out using a
promoter-less plasmid and subsequent measurement of luciferase activity in cells (1/30 of the positive control
containing SV40 promoter and enhancer).

Citations:

Martin M. M., Garcia J. A., McFarland J. D., Duffy A. A., Gregson J. P., Elton T. S. (2003) Translation of the human angiotensin II type 1 receptor mRNA is mediated by a highly efficient internal ribosome entry site. Mol. Cell. Endocrinol. 212(1-2):51-61

IRESs:

IRES:

Version: `4`	Last change: `2007-01-11 00:00:00`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `AT1R_var4` Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.

The IRES absolute position (the range includes START and STOP codons or their equivalents):

26-439

Conclusion:
putative_IRES

How IRES boundaries were determined:
guessed

The sequence of IRES region aligned to its secondary structure (if available):

ATTCCAGCGC CTGACAGCCA GGACCCCAGG CAGCAGCGAG TGACAGGACG TCTGGACCGG CGCGCCGCTA GCAGCTCTGC CGGGCCGCGG CGGTGATCGA 
^26        ^36        ^46        ^56        ^66        ^76        ^86        ^96        ^106       ^116       

TGGGGAGCGG CTGGAGCGGA CCCAGCGAGT GAGGGCGCAC AGCCGGGACG CCGAGGCGGC GGGCGGGAGA CCCGCACCAG CGCAGCCGGC CCTCGGCGGG 
^126       ^136       ^146       ^156       ^166       ^176       ^186       ^196       ^206       ^216       

ACGTGACGCA GCGCCCGGGG CGCGGGTTTG ATATTTGACA AATTGATCTA AAATGGCTGG GTTTTTATCT GAATAACTCA CTGATGCCAT CCCAGAAAGT 
^226       ^236       ^246       ^256       ^266       ^276       ^286       ^296       ^306       ^316       

CGGCACCAGA TGAAGAAAAT GAATCACAAG TCAACTGACA GTCCAAAGGC TCCACAGCTC AGAGGAGGTG TATTTGATAT AGTGTTTGCA ACAAATTCGA 
^326       ^336       ^346       ^356       ^366       ^376       ^386       ^396       ^406       ^416       

CCCAGGTGAT CAAA
^426       ^436

Remarks:

AGTR1 5' UTR from nt -414 to nt -1

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1