IRESite: The database of experimentally verified IRES structures

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Search IRESite database by a combination of any parameters

The checkbox in the leftmost column is automatically selected when you put any value into the respective field. If you check the checkbox while you leave empty the value field you will search for records which have the value empty. Thus, do not leave the checkbox crosschecked accidentally! Choose a boolean operator to combine more parameters together: AND  OR
	The IRESite_Id:
	The IRESite_Plasmid_Id:
	The IRESite_IRES_Id:
	The IRESite_IRES_2Dstruct_Id:
	The IRESite_2D_Exp_2Dstruct_Id:
	The IRESite_Seq_Id:
	The IRESite_IRES_Seq_Id:
	The IRESite_2D_Exp_Seq_Id:
	The IRESite_2D_Exp_Id:
	The GenBankId (GI:#):
	The name of the IRES:
	The name of the gene containing an IRES or name of the reporter gene used in some bicistronic construct:
	The function of the gene containing an IRES or the function of the reporter gene:
	Search by the number of ORFs present within some mRNA/+RNA: = != < <= >= >
	Search by the position of the START codon of an ORF: = != < <= >= >
	Search by the position of the STOP codon of an ORF: = != < <= >= >
	The type of the frameshift present within this ORF: 0 -1 +1 +2
	There exist alternatively initiated protein forms translated from the same mRNA/+RNA: no yes not tested
	Ribosome read-through a STOP codon is known in the mRNA/+RNA sequence: no yes
	Search by organism:
	Search by NCBI Taxonomy ID:
	Search by strain or the cell-line:
	The name of the donor gene containing an IRES:
	The promoter name:
	The plasmid name:
	The description of the mRNA/+RNA molecule or ORF or plasmid or ITAF or 2D structure:
	The recordtype: natural_transcript plasmid_with_promoter_and_putative_IRES_translationally_characterized plasmid_with_promoter_and_putative_IRES_without_translational_characterization negative_control_plasmid_with_promoter_and_without_putative_IRES promoter-less_plasmid_with_putative_IRES promoter-less_plasmid_without_putative_IRES
	The origin of the mRNA/+RNA molecule (use only when mRNA occurrence above == natural_transcript): nuclear cytoplasmatic mitochondrial plastidal plasmidal viral
	Search by origin of IRES inserted into the plasmid (use only when mRNA occurrence above == plasmid_with_promoter_and_putative_IRES_translationally_characterized): nuclear cytoplasmatic mitochondrial plastidal plasmidal viral engineered
	The completeness of the mRNA/+RNA sequence: possibly_wrong nonexisting_chimera_of_GenBank_record_and_tested_IRES_fragment only_IRES_fragment both_UTRs_incomplete 5UTR_incomplete 3UTR_incomplete both_UTRs_possibly_incomplete 5UTR_possibly_incomplete 3UTR_possibly_incomplete our_best_guess hopefully_full-length_mRNA end-to-end_full-length_mRNA
	The cDNA copy of the IRES region of mRNA/+RNA might have a promoter activity: yes no no (not convincing) not tested unclear
	The mRNA topology: linear circular
	The name/type of the rRNA complementary at least in some regions to some mRNA/+RNA present within IRESite:
	Our conclusion on existence/proof of the IRES: strongly_supported_IRES weakly_supported_IRES putative_IRES unproved_IRES probably_not_IRES disproved_IRES never_claimed_to_be_IRES
	The functional status of IRES segment in mRNA: functional defective
	Search by the size of IRES segment sequence: = != < <= >= >
	Search by the size of the nucleic acid (mRNA): = != < <= >= >
	Search by the size of the region in which its secondary structures were determined: = != < <= >= >
	Search by the position of the first (leftmost) base of IRES segment in mRNA/+RNA: = != < <= >= >
	Search by the position of the last (rightmost) base of IRES segment in mRNA/+RNA: = != < <= >= >
	Search by the position of the first (leftmost) base of IRES segment relative to the last (rightmost) base of the previous ORF: = != < <= >= >
	Search by the position of the last (rightmost) base of IRES segment relative to the last (rightmost) base of the previous ORF: = != < <= >= >
	Search by the position of the first (leftmost) base of IRES segment relative to the first (leftmost) base of the successive ORF: = != < <= >= >
	Search by the position of the last (rightmost) base of IRES segment relative to the first (leftmost) base of the successive ORF: = != < <= >= >
	1. The translation efficiency change in percentages of the new IRES: = != < <= >= >
	2. The translation efficiency change in percentages of the new IRES: = != < <= >= >
	1. The translation efficiency change in percentages of the negative control: = != < <= >= >
	2. The translation efficiency change in percentages of the negative control: = != < <= >= >
	The cap analogs affect the yield of the reporter proteins during translation: yes no not tested
	Whether supplemented rapamycin affects efficiency of translation: yes no not tested
	Characteristics of mRNA subject to translation: exogenous_RNA_with_GpppG_cap_with_polyA_tail exogenous_RNA_with_GpppG_cap_without_polyA_tail exogenous_RNA_with_ApppG_cap_with_polyA_tail exogenous_RNA_with_ApppG_cap_without_polyA_tail exogenous_RNA_without_cap_with_polyA_tail exogenous_RNA_without_cap_without_polyA_tail endogenous_nuclear_RNA_Pol_I_transcript endogenous_nuclear_RNA_Pol_II_transcript endogenous_nuclear_RNA_Pol_III_transcript endogenous_cytoplasmic_uncapped_T7_transcript_without_polyA_tail endogenous_cytoplasmic_uncapped_T7_transcript_with_polyA_tail
	The translation method: in vivo in vitro
	Type of the in vitro translation system used to translate mRNA/+RNA: rabbit reticulocytes lysate wheat germ lysate HeLa cell lysate SF268 cell lysate (human glioblastoma) yeast lysate frog oocytes cell-free Drosophila-embryo lysate primary cultured neurons Sf21 cell-based lysate system other
	Find records with known secondary structures of the respective mRNA/+RNA:
	Select the checkbox whether any proteins are known to interact with the respective mRNA/+RNA. This also includes trans-acting protein factors (ITAFs) known to modulate IRES activity.
	Description/name of either ITAF or interacting protein:
	The remarks text contains the following substring: