IRESite_Id:309 characteristics of GBV-B IRES studied in RepABla plasmid

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `309`	Version: `1`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš	Last change: `2008-06-04 21:42:05`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
hopefully_full-length_mRNA

The mRNA/+RNA description:

In vitro bicistronic transcript (non-capped non-poly(A)-tailed) containing
5' -GBV-B_IRES - beta-lactamase - EMCV-R_IRES - NS3-NS5B_GBV-B_polyprotein - 3'

The mRNA/+RNA sequence represented in the +DNA notation:

Gaccacaaac actccagttt gttacactcc gctaggaatg ctcctggagc acccccccta gcagggcgtg ggggatttcc cctgcccgtc tgcagaaggg 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tggagccaac caccttagta tgtaggcggc gggactcatg acgctcgcgt gatgacaagc gccaagcttg acttggatgg ccctgatggg cgttcatggg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

ttcggtggtg gtggcgcttt aggcagcctc cacgcccacc acctcccaga tagagcggcg gcactgtagg gaagaccggg gaccggtcac taccaaggac 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gcagacctct ttttgagtat cacgcctccg gaagtagttg ggcaagccca cctatatgtg ttgggatggt tggggttagc catccatacc gtactgcctg 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

atagggtcct tgcgagggga tctgggagtc tcgtagaccg tagcacatgg ggcgcgccat gcacccagaa acgctggtga aagtaaaaga tgctgaagat 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taagtttaaa cagaccacaa cggtttccct ctagcgggat caattccgcc 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggcacc ttttacgctg cagtgtctct 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

ctgaacgtgg cacgctgtca gcgatggcag tggtcatgac tggtatagac ccccgaactt ggactggaac tatcttcaga ttaggatctc tggccactag 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ctacatggga tttgtttgtg acaacgtgtt gtatactgct caccatggca gcaaggggcg ccggttggct catcccacag gctccataca cccaataacc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gttgacgcgg ctaatgacca ggacatctat caaccaccat gtggagctgg gtcccttact cggtgctctt gcggggagac caaggggtat ctggtaacac 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

gactggggtc attggttgag gtcaataaat ccgatgaccc ttattggtgt gtgtgcgggg cccttcccat ggctgttgcc aagggttctt caggtgcccc 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

gattctgtgc tcctccgggc atgttattgg gatgttcacc gctgctagaa attctggcgg ttcagtcagc cagattaggg ttaggccgtt ggtgtgtgct 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

ggataccatc cccagtacac agcacatgcc actcttgata caaaacctac tgtgcctaac gagtattcag tgcaaatttt aattgccccc actggcagcg 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

gcaagtcaac caaattacca ctttcttaca tgcaggagaa gtatgaggtc ttggtcctaa atcccagtgt ggctacaaca gcatcaatgc caaagtacat 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

gcacgcgacg tacggcgtga atccaaattg ctattttaat ggcaaatgta ccaacacagg ggcttcactt acgtacagca catatggcat gtacctgacc 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ggagcatgtt cccggaacta tgatgtaatc atttgtgacg aatgccatgc taccgatgca accaccgtgt tgggcattgg aaaggtccta accgaagctc 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

catccaaaaa tgttaggcta gtggttcttg ccacggctac cccccctgga gtaatcccta caccacatgc caacataact gagattcaat taaccgatga 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

aggcactatc ccctttcatg gaaaaaagat taaggaggaa aatctgaaga aagggagaca ccttatcttt gaggctacca aaaaacactg tgatgagctt 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

gctaacgagt tagctcgaaa gggaataaca gctgtctctt actatagggg atgtgacatc tcaaaaatcc ctgagggcga ctgtgtagta gttgccactg 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

atgccttgtg tacagggtac actggtgact ttgattccgt gtatgactgc agcctcatgg tagaaggcac atgccatgtt gaccttgacc ctactttcac 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

catgggtgtt cgtgtgtgcg gggtttcagc aatagttaaa ggccagcgta ggggccgcac aggccgtggg agagctggca tatactacta tgtagacggg 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

agttgtaccc cttcgggtat ggttcctgaa tgcaacattg ttgaagcctt cgacgcagcc aaggcatggt atggtttgtc atcaacagaa gctcaaacta 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

ttctggacac ctatcgcacc caacctgggt tacctgcgat aggagcaaat ttggacgagt gggctgatct cttttcaatg gtcaaccccg aaccttcatt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tgtcaatact gcaaaaagaa ctgctgacaa ttatgttttg ttgactgcag cccaactaca actgtgtcat cagtatggct atgctgctcc caatgacgca 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

ccacggtggc agggagcccg gcttgggaaa aaaccttgtg gggttctgtg gcgcttggac ggcgctgacg cctgtcctgg cccagagccc agcgaggtga 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ccagatacca aatgtgcttc actgaagtca atacttctgg gacagccgca ctcgctgttg gcgttggagt ggctatggct tatctagcca ttgacacttt 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

tggcgccact tgtgtgcggc gttgctggtc tattacatca gtccctaccg gtgctactgt cgccccagtg gttgacgaag aagaaatcgt ggaggagtgt 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

gcatcattca ttcccttgga ggccatggtt gctgcaattg acaagctgaa gagtacaatc accacaacta gtcctttcac attggaaacc gcccttgaaa 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

aacttaacac ctttcttggg cctcatgcag ctacaatcct tgctatcata gagtattgct gtggcttagt cactttacct gacaatccct ttgcatcatg 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

cgtgtttgct ttcattgcgg gtattactac cccactacct cacaagatca aaatgttcct gtcattattt ggaggcgcaa ttgcgtccaa gcttacagac 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

gctagaggcg cactggcgtt catgatggcc ggggctgcgg gaacagctct tggtacatgg acatcggtgg gttttgtctt tgacatgcta ggcggctatg 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ctgccgcctc atccactgct tgcttgacat ttaaatgctt gatgggtgag tggcccacta tggatcagct tgctggttta gtctactccg cgttcaaccc 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

ggccgcagga gttgtgggcg ttttgtcagc ttgtgcaatg tttgctttga caacagcagg gccagatcac tggcccaaca gacttcttac tatgcttgct 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

aggagcaaca ctgtatgtaa tgagtacttt attgccactc gtgacatccg caggaagata ctgggcattc tggaggcatc taccccctgg agtgtcatat 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

cagcttgcat ccgttggctc cacaccccga cggaggatga ttgcggcctc attgcttggg gtctagagat ttggcagtac gtgtgcaatt tctttgtgat 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

ttgctttaat gtccttaaag ctggagttca gagcatggtt aacattcctg gttgtccttt ctacagctgc cagaaggggt acaagggccc ctggattgga 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

tcaggtatgc tccaagcacg ctgtccatgc ggtgctgaac tcatcttttc tgttgagaat ggttttgcaa aactttacaa aggacccaga acttgttcaa 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

attactggag aggggctgtt ccagtcaacg ctaggctgtg tgggtcggct agaccggacc caactgattg gactagtctt gtcgtcaatt atggcgttag 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

ggactactgt aaatatgaga aattgggaga tcacatcttt gttacagcag tatcctctcc aaatgtctgt ttcacccagg tgcccccaac cttgagagct 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

gcagtggccg tggacggcgt acaggttcag tgttatctag gtgagcccaa aactccttgg acgacatctg cttgctgtta cggtcctgac ggtaagggta 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

aaactgttaa gcttcccttc cgcgttgacg gtcacacacc tggtgtgcgc atgcaactta atttgcgtga tgcacttgag acaaatgact gtaattccac 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

aaacaacact cctagtgatg aagccgcagt gtccgctctt gttttcaaac aggagttgcg gcgtacaaac caattgcttg aggcaatttc agctggcgtt 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

gacaccacca aactgccagc cccctccatc gaagaggtag tggtaagaaa gcgccagttc cgggcaagaa ctggttcgct taccttgccc ccccctccga 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

gatccgtccc aggagtgtca tgtcctgaaa gcctgcaacg aagtgacccg ttagaaggtc cttcaaacct ccctccttca ccacctgttc tacagttggc 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

catgccgatg cccctgttgg gagcgggtga gtgtaaccct ttcactgcaa ttggatgtgc aatgaccgaa acaggcggag gccctgatga tttacccagt 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

taccctccca aaaaggaggt ctctgaatgg tcagacgaaa gttggtcaac ggctacaacc gcttccagct acgttactgg ccccccgtac cctaagatac 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ggggaaagga ttccactcag tcagcccccg ccaaacggcc tacaaaaaag aagttgggaa agagtgagtt ttcgtgcagc atgagctaca cttggaccga 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

cgtgattagc ttcaaaactg cttctaaagt tctgtctgca actcgggcca tcactagtgg tttcctcaaa caaagatcat tggtgtatgt gactgagccg 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

cgggatgcgg agcttagaaa acaaaaagtc actattaata gacaacctct gttcccccca tcataccaca agcaagtgag attggctaag gagaaagctt 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

caaaagttgt cggtgtcatg tgggactatg atgaagtagc agctcacacg ccctctaagt ctgctaagtc ccacatcact ggccttcggg gcactgatgt 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

tcgttctgga gcagcccgca aggctgttct ggacttgcag aagtgtgtcg aggcaggtga gataccgagt cattatcggc aaacagtgat agttccaaag 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

gaggaggtct tcgtgaagac cccccagaaa ccaacaaaga aacccccaag gctcatctcg tacccccacc ttgaaatgag atgtgttgag aagatgtact 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

acggtcaggt tgctcctgac gtagttaaag ctgtcatggg agatgcgtac gggtttgtag atccacgtac ccgtgtcaag cgtctgttgt cgatgtggtc 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

acccgatgca gtcggagcca catgcgatac agtgtgtttt gacagtacca tcacacccga ggatatcatg gtggagacag acatctactc agcagctaaa 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

ctcagtgacc aacaccgagc tggcattcac accattgcga ggcagttata cgctggagga ccgatgatcg cttatgatgg ccgagagatc ggatatcgta 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

ggtgtaggtc ttccggcgtc tatactacct caagttccaa cagtttgacc tgctggctga aggtaaatgc tgcagccgaa caggctggca tgaagaaccc 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

tcgcttcctt atttgcggcg atgattgcac cgtaatttgg aaaagcgccg gagcagatgc agacaaacaa gcaatgcgtg tctttgctag ctggatgaag 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

gtgatgggtg caccacaaga ttgtgtgcct caacccaaat acagtttgga agaattaaca tcatgctcat caaatgttac ctctggaatt accaaaagtg 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

gcaagcctta ctactttctt acaagagatc ctcgtatccc ccttggcagg tgctctgccg agggtctggg atacaacccc agtgctgcgt ggattgggta 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

tctaatacat cactacccat gtttgtgggt tagccgtgtg ttggctgtcc atttcatgga gcagatgctc tttgaggaca aacttcccga gactgtgacc 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

tttgactggt atgggaaaaa ttatacggtg cctgtagaag atctgcccag catcattgct ggtgtgcacg gtattgaggc tttctcggtg gtgcgctaca 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

ccaacgctga gatcctcaga gtttcccaat cactaacaga catgaccatg ccccccctgc gagcctggcg aaagaaagcc agggcggtcc tcgccagcgc 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

caagaggcgt ggcggagcac acgcaaaatt ggctcgcttc cttctctggc atgctacatc tagacctcta ccagatttgg ataagacgag cgtggctcgg 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

tacaccactt tcaattattg tgatgtttac tccccggagg gggatgtgtt tgttacacca cagagaagat tgcagaagtt tcttgtgaag tatttggctg 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

tcattgtttt tgccctaggg ctcattgctg ttggattagc catcagctga acccccaaat tcaaaattaa ctaacagttt tttttttttt ttttttttag 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

ggcagcggca acaggggaga ccccgggctt aacgaccccg ccgatgtgag tttggcgacc atggtggatc agaaccgttt cgggtgaagc catggtctga 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

aggggatgac gtcccttctg gctcatccac aaaaaccgtc tcgggtgggt gaggagtcct ggctgtgtgg gaagcagtca gtataattcc cgtcgtgtgt 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

ggtgacgcct cacgacgtac ttgtccgctg tgcagagcgt agtaccaagg gctgcacccc ggtttttgtt ccaagcggag ggcaaccccc gcttggaatt 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

aaaaact
^8001

Credibility of mRNA sequence:

end-to-end_sequence_reverse_engineered_and_should_match_experiment

The name of the plasmid:
RepABla The name of the promoter used to express this mRNA:
T7

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
GBV-B

The origin of IRES in the plasmid:

viral

The donor organism of the IRES segment:

Hepatitis GB virus B ACY/GBV-B/FL-3

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

ggatccggta aTACGACTCA CTATAGacca caaacactcc agtttgttac actccgctag gaatgctcct ggagcacccc ccctagcagg gcgtggggga 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tttcccctgc ccgtctgcag aagggtggag ccaaccacct tagtatgtag gcggcgggac tcatgacgct cgcgtgatga caagcgccaa gcttgacttg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

gatggccctg atgggcgttc atgggttcgg tggtggtggc gctttaggca gcctccacgc ccaccacctc ccagatagag cggcggcact gtagggaaga 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

ccggggaccg gtcactacca aggacgcaga cctctttttg agtatcacgc ctccggaagt agttgggcaa gcccacctat atgtgttggg atggttgggg 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ttagccatcc ataccgtact gcctgatagg gtccttgcga ggggatctgg gagtctcgta gaccgtagca catggggcgc gccatgcacc cagaaacgct 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

attctcagaa tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaagt ttaaacagac cacaacggtt 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

tccctctagc gggatcaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

gcacctttta cgctgcagtg tctctctgaa cgtggcacgc tgtcagcgat ggcagtggtc atgactggta tagacccccg aacttggact ggaactatct 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

tcagattagg atctctggcc actagctaca tgggatttgt ttgtgacaac gtgttgtata ctgctcacca tggcagcaag gggcgccggt tggctcatcc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

cacaggctcc atacacccaa taaccgttga cgcggctaat gaccaggaca tctatcaacc accatgtgga gctgggtccc ttactcggtg ctcttgcggg 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

gagaccaagg ggtatctggt aacacgactg gggtcattgg ttgaggtcaa taaatccgat gacccttatt ggtgtgtgtg cggggccctt cccatggctg 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ttgccaaggg ttcttcaggt gccccgattc tgtgctcctc cgggcatgtt attgggatgt tcaccgctgc tagaaattct ggcggttcag tcagccagat 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

tagggttagg ccgttggtgt gtgctggata ccatccccag tacacagcac atgccactct tgatacaaaa cctactgtgc ctaacgagta ttcagtgcaa 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

attttaattg cccccactgg cagcggcaag tcaaccaaat taccactttc ttacatgcag gagaagtatg aggtcttggt cctaaatccc agtgtggcta 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

caacagcatc aatgccaaag tacatgcacg cgacgtacgg cgtgaatcca aattgctatt ttaatggcaa atgtaccaac acaggggctt cacttacgta 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

cagcacatat ggcatgtacc tgaccggagc atgttcccgg aactatgatg taatcatttg tgacgaatgc catgctaccg atgcaaccac cgtgttgggc 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

attggaaagg tcctaaccga agctccatcc aaaaatgtta ggctagtggt tcttgccacg gctacccccc ctggagtaat ccctacacca catgccaaca 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

taactgagat tcaattaacc gatgaaggca ctatcccctt tcatggaaaa aagattaagg aggaaaatct gaagaaaggg agacacctta tctttgaggc 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

taccaaaaaa cactgtgatg agcttgctaa cgagttagct cgaaagggaa taacagctgt ctcttactat aggggatgtg acatctcaaa aatccctgag 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

ggcgactgtg tagtagttgc cactgatgcc ttgtgtacag ggtacactgg tgactttgat tccgtgtatg actgcagcct catggtagaa ggcacatgcc 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

atgttgacct tgaccctact ttcaccatgg gtgttcgtgt gtgcggggtt tcagcaatag ttaaaggcca gcgtaggggc cgcacaggcc gtgggagagc 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

tggcatatac tactatgtag acgggagttg taccccttcg ggtatggttc ctgaatgcaa cattgttgaa gccttcgacg cagccaaggc atggtatggt 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

ttgtcatcaa cagaagctca aactattctg gacacctatc gcacccaacc tgggttacct gcgataggag caaatttgga cgagtgggct gatctctttt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

caatggtcaa ccccgaacct tcatttgtca atactgcaaa aagaactgct gacaattatg ttttgttgac tgcagcccaa ctacaactgt gtcatcagta 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

tggctatgct gctcccaatg acgcaccacg gtggcaggga gcccggcttg ggaaaaaacc ttgtggggtt ctgtggcgct tggacggcgc tgacgcctgt 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

cctggcccag agcccagcga ggtgaccaga taccaaatgt gcttcactga agtcaatact tctgggacag ccgcactcgc tgttggcgtt ggagtggcta 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

tggcttatct agccattgac acttttggcg ccacttgtgt gcggcgttgc tggtctatta catcagtccc taccggtgct actgtcgccc cagtggttga 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

cgaagaagaa atcgtggagg agtgtgcatc attcattccc ttggaggcca tggttgctgc aattgacaag ctgaagagta caatcaccac aactagtcct 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

ttcacattgg aaaccgccct tgaaaaactt aacacctttc ttgggcctca tgcagctaca atccttgcta tcatagagta ttgctgtggc ttagtcactt 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

tacctgacaa tccctttgca tcatgcgtgt ttgctttcat tgcgggtatt actaccccac tacctcacaa gatcaaaatg ttcctgtcat tatttggagg 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

cgcaattgcg tccaagctta cagacgctag aggcgcactg gcgttcatga tggccggggc tgcgggaaca gctcttggta catggacatc ggtgggtttt 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

gtctttgaca tgctaggcgg ctatgctgcc gcctcatcca ctgcttgctt gacatttaaa tgcttgatgg gtgagtggcc cactatggat cagcttgctg 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

gtttagtcta ctccgcgttc aacccggccg caggagttgt gggcgttttg tcagcttgtg caatgtttgc tttgacaaca gcagggccag atcactggcc 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

caacagactt cttactatgc ttgctaggag caacactgta tgtaatgagt actttattgc cactcgtgac atccgcagga agatactggg cattctggag 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

gcatctaccc cctggagtgt catatcagct tgcatccgtt ggctccacac cccgacggag gatgattgcg gcctcattgc ttggggtcta gagatttggc 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

agtacgtgtg caatttcttt gtgatttgct ttaatgtcct taaagctgga gttcagagca tggttaacat tcctggttgt cctttctaca gctgccagaa 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

ggggtacaag ggcccctgga ttggatcagg tatgctccaa gcacgctgtc catgcggtgc tgaactcatc ttttctgttg agaatggttt tgcaaaactt 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

tacaaaggac ccagaacttg ttcaaattac tggagagggg ctgttccagt caacgctagg ctgtgtgggt cggctagacc ggacccaact gattggacta 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

gtcttgtcgt caattatggc gttagggact actgtaaata tgagaaattg ggagatcaca tctttgttac agcagtatcc tctccaaatg tctgtttcac 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ccaggtgccc ccaaccttga gagctgcagt ggccgtggac ggcgtacagg ttcagtgtta tctaggtgag cccaaaactc cttggacgac atctgcttgc 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

tgttacggtc ctgacggtaa gggtaaaact gttaagcttc ccttccgcgt tgacggtcac acacctggtg tgcgcatgca acttaatttg cgtgatgcac 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

ttgagacaaa tgactgtaat tccacaaaca acactcctag tgatgaagcc gcagtgtccg ctcttgtttt caaacaggag ttgcggcgta caaaccaatt 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

gcttgaggca atttcagctg gcgttgacac caccaaactg ccagccccct ccatcgaaga ggtagtggta agaaagcgcc agttccgggc aagaactggt 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

tcgcttacct tgcccccccc tccgagatcc gtcccaggag tgtcatgtcc tgaaagcctg caacgaagtg acccgttaga aggtccttca aacctccctc 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

cttcaccacc tgttctacag ttggccatgc cgatgcccct gttgggagcg ggtgagtgta accctttcac tgcaattgga tgtgcaatga ccgaaacagg 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

cggaggccct gatgatttac ccagttaccc tcccaaaaag gaggtctctg aatggtcaga cgaaagttgg tcaacggcta caaccgcttc cagctacgtt 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

actggccccc cgtaccctaa gatacgggga aaggattcca ctcagtcagc ccccgccaaa cggcctacaa aaaagaagtt gggaaagagt gagttttcgt 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

gcagcatgag ctacacttgg accgacgtga ttagcttcaa aactgcttct aaagttctgt ctgcaactcg ggccatcact agtggtttcc tcaaacaaag 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

atcattggtg tatgtgactg agccgcggga tgcggagctt agaaaacaaa aagtcactat taatagacaa cctctgttcc ccccatcata ccacaagcaa 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

gtgagattgg ctaaggagaa agcttcaaaa gttgtcggtg tcatgtggga ctatgatgaa gtagcagctc acacgccctc taagtctgct aagtcccaca 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

tcactggcct tcggggcact gatgttcgtt ctggagcagc ccgcaaggct gttctggact tgcagaagtg tgtcgaggca ggtgagatac cgagtcatta 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

tcggcaaaca gtgatagttc caaaggagga ggtcttcgtg aagacccccc agaaaccaac aaagaaaccc ccaaggctca tctcgtaccc ccaccttgaa 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

atgagatgtg ttgagaagat gtactacggt caggttgctc ctgacgtagt taaagctgtc atgggagatg cgtacgggtt tgtagatcca cgtacccgtg 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

tcaagcgtct gttgtcgatg tggtcacccg atgcagtcgg agccacatgc gatacagtgt gttttgacag taccatcaca cccgaggata tcatggtgga 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

gacagacatc tactcagcag ctaaactcag tgaccaacac cgagctggca ttcacaccat tgcgaggcag ttatacgctg gaggaccgat gatcgcttat 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

gatggccgag agatcggata tcgtaggtgt aggtcttccg gcgtctatac tacctcaagt tccaacagtt tgacctgctg gctgaaggta aatgctgcag 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

ccgaacaggc tggcatgaag aaccctcgct tccttatttg cggcgatgat tgcaccgtaa tttggaaaag cgccggagca gatgcagaca aacaagcaat 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

gcgtgtcttt gctagctgga tgaaggtgat gggtgcacca caagattgtg tgcctcaacc caaatacagt ttggaagaat taacatcatg ctcatcaaat 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

gttacctctg gaattaccaa aagtggcaag ccttactact ttcttacaag agatcctcgt atcccccttg gcaggtgctc tgccgagggt ctgggataca 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

accccagtgc tgcgtggatt gggtatctaa tacatcacta cccatgtttg tgggttagcc gtgtgttggc tgtccatttc atggagcaga tgctctttga 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

ggacaaactt cccgagactg tgacctttga ctggtatggg aaaaattata cggtgcctgt agaagatctg cccagcatca ttgctggtgt gcacggtatt 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

gaggctttct cggtggtgcg ctacaccaac gctgagatcc tcagagtttc ccaatcacta acagacatga ccatgccccc cctgcgagcc tggcgaaaga 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

aagccagggc ggtcctcgcc agcgccaaga ggcgtggcgg agcacacgca aaattggctc gcttccttct ctggcatgct acatctagac ctctaccaga 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

tttggataag acgagcgtgg ctcggtacac cactttcaat tattgtgatg tttactcccc ggagggggat gtgtttgtta caccacagag aagattgcag 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

aagtttcttg tgaagtattt ggctgtcatt gtttttgccc tagggctcat tgctgttgga ttagccatca gctgaacccc caaattcaaa attaactaac 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

agtttttttt tttttttttt tttagggcag cggcaacagg ggagaccccg ggcttaacga ccccgccgat gtgagtttgg cgaccatggt ggatcagaac 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

cgtttcgggt gaagccatgg tctgaagggg atgacgtccc ttctggctca tccacaaaaa ccgtctcggg tgggtgagga gtcctggctg tgtgggaagc 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

agtcagtata attcccgtcg tgtgtggtga cgcctcacga cgtacttgtc cgctgtgcag agcgtagtac caagggctgc accccggttt ttgttccaag 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

cggagggcaa cccccgcttg gaattaaaaa cttgaagagc ctcgaggaaa aatcagtaga aatagctgtt ccagtctttc tagccttgat tccacttctg 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

tcagatgtgc cctagtcagc ggagaccttt tggttttggg agagtagcga cactcccagt tgttcttcag acacttggcg cacttcggtt tttctttgga 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

gcacttgagc tttttaagtc ggcaaatatc gcatgcttgt tcgatagaag acagtagctt catctttcag gaggcttgct tcaagcttgg agttgattgt 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

atgcttggta tagcttgaaa tattgtgcag aaaaagaaac aaggaagaaa gggaacgaga acaatgacga ggaaacaaaa gattaataat tgcaggtcta 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

tttatacttg atagcaaagc ggcaaacttt ttttatttca aattcaagta actggaagga aggccgtata ccgttgctca ttagagagta gtgtgcgtga 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

atgaaggaag gaaaaagttt cgtgtgttcg aagatacccc tcatcagctc tggaacaacg acatctgttg gtgctgtctt tgtcgttaat tttttccttt 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

agtgtcttcc atcatttttt ttgtcattgc ggatatggtg agacaacaac gggggagaga gaaaagaaaa aaaaagaaaa gaagttgcat gcaagcaacg 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg ttatggtgca ctctcagtac aatctgctct gatgccgcat 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981     ^10991     

aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
^11001     ^11011     ^11021     ^11031     ^11041     ^11051     ^11061     ^11071     ^11081     ^11091     

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
^11101     ^11111     ^11121     ^11131     ^11141     ^11151     ^11161     ^11171     ^11181     ^11191     

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
^11201     ^11211     ^11221     ^11231     ^11241     ^11251     ^11261     ^11271     ^11281     ^11291     

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
^11301     ^11311     ^11321     ^11331     ^11341     ^11351     ^11361     ^11371     ^11381     ^11391     

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
^11401     ^11411     ^11421     ^11431     ^11441     ^11451     ^11461     ^11471     ^11481     ^11491     

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
^11501     ^11511     ^11521     ^11531     ^11541     ^11551     ^11561     ^11571     ^11581     ^11591     

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
^11601     ^11611     ^11621     ^11631     ^11641     ^11651     ^11661     ^11671     ^11681     ^11691     

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
^11701     ^11711     ^11721     ^11731     ^11741     ^11751     ^11761     ^11771     ^11781     ^11791     

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
^11801     ^11811     ^11821     ^11831     ^11841     ^11851     ^11861     ^11871     ^11881     ^11891     

gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 
^11901     ^11911     ^11921     ^11931     ^11941     ^11951     ^11961     ^11971     ^11981     ^11991     

gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc 
^12001     ^12011     ^12021     ^12031     ^12041     ^12051     ^12061     ^12071     ^12081     ^12091     

cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg caccccaggc tttacacttt 
^12101     ^12111     ^12121     ^12131     ^12141     ^12151     ^12161     ^12171     ^12181     ^12191     

atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attaattcgg tcgaaaaaag 
^12201     ^12211     ^12221     ^12231     ^12241     ^12251     ^12261     ^12271     ^12281     ^12291     

aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat 
^12301     ^12311     ^12321     ^12331     ^12341     ^12351     ^12361     ^12371     ^12381     ^12391     

ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta 
^12401     ^12411     ^12421     ^12431     ^12441     ^12451     ^12461     ^12471     ^12481     ^12491     

tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata 
^12501     ^12511     ^12521     ^12531     ^12541     ^12551     ^12561     ^12571     ^12581     ^12591     

cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg 
^12601     ^12611     ^12621     ^12631     ^12641     ^12651     ^12661     ^12671     ^12681     ^12691     

caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt 
^12701     ^12711     ^12721     ^12731     ^12741     ^12751     ^12761     ^12771     ^12781     ^12791     

cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag 
^12801     ^12811     ^12821     ^12831     ^12841     ^12851     ^12861     ^12871     ^12881     ^12891     

cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag 
^12901     ^12911     ^12921     ^12931     ^12941     ^12951     ^12961     ^12971     ^12981     ^12991     

acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca 
^13001     ^13011     ^13021     ^13031     ^13041     ^13051     ^13061     ^13071     ^13081     ^13091     

cttgccggca tgccggtaga ggtgtggtca ataagagcga cctcatgcta tacctgagaa agcaacctga cctacaggaa agagttactc aagaacaaga 
^13101     ^13111     ^13121     ^13131     ^13141     ^13151     ^13161     ^13171     ^13181     ^13191     

attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc 
^13201     ^13211     ^13221     ^13231     ^13241     ^13251     ^13261     ^13271     ^13281     ^13291     

ccggaattag cttggctgca ggtcgac
^13301     ^13311     ^13321

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
bla

The description of the protein encoded in this ORF:
beta-lactamase fusion protein

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

447-1253

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
polyprotein

The description of the protein encoded in this ORF:
NS3-4A, NS4B, NS5A, NS5B polyprotein

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1873-7650

Citations:

Pizzuti M., De Tomassi A., Traboni C. (2003) Replication and IRES-dependent translation are both affected by core coding sequences in subgenomic GB virus B replicons. J. Virol. 77(13):7502-7509

IRESs:

IRES:

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `GBV-B`	The functional status of IRES: `defective`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

2-446

How IRES boundaries were determined:
experimentally_determined

The sequence of IRES region aligned to its secondary structure (if available):

accacaaaca ctccagtttg ttacactccg ctaggaatgc tcctggagca ccccccctag cagggcgtgg gggatttccc ctgcccgtct gcagaagggt 
^2         ^12        ^22        ^32        ^42        ^52        ^62        ^72        ^82        ^92        

ggagccaacc accttagtat gtaggcggcg ggactcatga cgctcgcgtg atgacaagcg ccaagcttga cttggatggc cctgatgggc gttcatgggt 
^102       ^112       ^122       ^132       ^142       ^152       ^162       ^172       ^182       ^192       

tcggtggtgg tggcgcttta ggcagcctcc acgcccacca cctcccagat agagcggcgg cactgtaggg aagaccgggg accggtcact accaaggacg 
^202       ^212       ^222       ^232       ^242       ^252       ^262       ^272       ^282       ^292       

cagacctctt tttgagtatc acgcctccgg aagtagttgg gcaagcccac ctatatgtgt tgggatggtt ggggttagcc atccataccg tactgcctga 
^302       ^312       ^322       ^332       ^342       ^352       ^362       ^372       ^382       ^392       

tagggtcctt gcgaggggat ctgggagtct cgtagaccgt agcac
^402       ^412       ^422       ^432       ^442

Citations:

Pizzuti M., De Tomassi A., Traboni C. (2003) Replication and IRES-dependent translation are both affected by core coding sequences in subgenomic GB virus B replicons. J. Virol. 77(13):7502-7509

The translation experiments:

Translation results:

IRESite Translation Id: 395

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vitro The in vitro translation system:
rabbit reticulocytes lysate

The organism used for translation:

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

3.700

Name of IRES used as the positive control:

GBV-B

Name of the plasmid used as the positive control. `RepDBla`
`IRESite Id` of the plasmid used as positive control. `306`
The relative translation efficiency in % of the positive control: `100.000`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

exogenous_RNA_without_cap_without_polyA_tail

Remarks:

The constructs had the putative GBV-B IRES placed in front of the 1st cistron and EMCV IRES placed in the
intercistronic region and therefore it does not make sense to consider size of the intercistronic region
in respect to GBV-B IRES.

Citations:

Pizzuti M., De Tomassi A., Traboni C. (2003) Replication and IRES-dependent translation are both affected by core coding sequences in subgenomic GB virus B replicons. J. Virol. 77(13):7502-7509

Last change to the database: 2019-03-18 09:32:49 GMT+1