IRESite record type: plasmid_with_promoter_and_putative_IRES_without_translational_characterization
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: hopefully_full-length_mRNA
The mRNA/+RNA description:
Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_DAP5/CAT which
comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron
respectively and the part of human DAP5 5' UTR (nt from -1 to nt -305 of the original sequence) mRNA
cloned between them in reverse complement orientation.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The name of the promoter used to express this mRNA: CMV
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing part of human DAP5 5' UTR (nt from -1 to nt -305 of the original sequence) inserted between
beta galactosidase and chloramphenicol acetyltransferase reporter genes in reverse complement orientation.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: DAP5
The description of the protein encoded in this ORF: chloramphenicol acetyltransferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 3980-4639
Remarks:
The plasmid was used as the negative control for experiments in article (Nevis et al., 2003; Distinct
Regulation of Internal Ribosome Entry Site-mediated Translation following Cellular Stress Is Mediated by
Apoptotic Fragments of eIF4G Translation Initiation Factor Family Members eIF4GI and p97/DAP5/NAT1
(PMID:12458215).
The IRES absolute position (the range includes START and STOP codons or their equivalents): 3621-3925
How IRES boundaries were determined: experimentally_determined
5'-end of IRES relative to last base of the STOP codon of the upstream ORF: 289
3'-end of IRES relative to last base of the STOP codon of the upstream ORF: 593
5'-end of IRES relative to first base of the START codon of the downstream ORF: -359
3'-end of IRES relative to first base of the START codon of the downstream ORF: -55
The sequence of IRES region aligned to its secondary structure (if available):
Remarks:
rc_DAP5 IRES represents the part of the human DAP5 5' UTR (nt from -1 to nt -305) cloned in the pbetaGAL/CAT
plasmid in reverse complement orientation.